Molecular Biology Course Page--FALL 2012 (H. Super)

 Minot State University

Biol 480/ Molecular Biology Course Page

Fall 2012

ANNOUNCEMENTS

 

Check out this awesome resourse:  DNA Learning Center

More terrific animations here!

 

 

LECTURE MATERIALS

Syllabus

General expectations

Powerpoint lectures:

Lecture 35

Lectute 36

Lecture 37--will bring to class

Lecture 38

 

 

ANIMATIONS

The Meselson-Stahl Experiment

Animation   Essential Biochemistry - DNA Replication

DNA replication 2

Transcription

LAB MATERIALS

Lab syllabus: here

Lab 1--Bacterial Transformation/DNA IS the genetic material

Lab 1 discussion--be sure to have gone over plates from lab 1 before you come to lab for experiement 2.  I expect group discussion!

Lab 2--PGLO plasmid purification

Lab 3--Using Spectrophotometry to analyze plasmid DNA/Setting up restriction digests of plamid

Lab 4--Analysis of restriction fragments

See your electrophoresis results here.

Lab 5--purification of GFP

Lab 6--Bioinformatics/PCR primer design---I will bring printed information. Please bring your own computer if possible.  

pGLO sequenc

Lab 7--SDS-PAGE

Lab 8--Endpoint PCR

Lab 8 part 2 agarose gel

Lab 9  real-time PCR

Lab 9--analysis (additional graphs will be given in lab)

 

 

 

Good website for GFP info:http://www.conncoll.edu/ccacad/zimmer/GFP-ww/GFP-1.htm

 

 

 

Exam topics lists

exam 1 topics

exam 2 topics

Exam 3 topics list

OLD MATERIAL BELOW THIS LINE

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Lecture handouts week of 2-22-10 molecular documents week 3-22-10

 

lab 7 posted below

 

Total points at mid term: 222

LECTURE MATERIAL

topics list topicsexam2

molecular 2010 syllabus

Molecules of the cell--Reading

GFP readings

lactoferrin gfp  pGLO lactoferrin

walnut GFP.jpg  walnut GFP

 

 

LAB MATERIAL

Syllabus  MOLECULAR LAB.-lab 2010

Lab 1-- PDF lab1   or Word doc lab1transformation2010

Lab 2Lab 2 plasmid pure

Lab3lab3plasmidanalysis

Lab 4lab 4 plasmidanalysis-week 2.doc

Lab 5lab 5 gfp purification

protein gel picture

-Native gel picture from previous  year's class.  Lane 1 is protein marker, the rest of the lanes are lysates from E. coli transformants. Our gel did not run far enough to see many bands or to resolve the molecular weight marker.

protein gel

Lab 6molecular lab 6 2010 part 1

Lab 6 part 2molecular lab 6 2010 part 2

Lab 7lab7 real time PCR

 

 

 

 

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ANIMATIONS

The Meselson-Stahl Experiment

Animation   Essential Biochemistry - DNA Replication

Protein Synthesis Animation

Semi-conservative replication animation

 

DnaTube.com link here--check out protein folding!

DnaTube.com Scientific Video Site

Telomerase demos

Telomerase!

Horn Lab - Projects

 

FORMS

lecuture summary page

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Biol4802012lecture 27.ppt3.02 MB