Molecular Biology Course Page--FALL 2012 (H. Super)

 Minot State University

Biol 480/ Molecular Biology Course Page

Fall 2014

 

ANNOUNCEMENTS

 

Check out these awesome resourses:  

DNA Learning Center

IFL science :)

 

LECTURE MATERIALS

Syllabus 2014

General expectations

Powerpoint lectures:

Lecture 27

Lecture 28

Lecture 29

Lecture 30

Lecture 31

Lecture 32

Lecture 33

Lecture 34

 

 

Topics Lists for Exams

Topics 1

Topics 2

Topics 3

 

LAB MATERIALS

Lab syllabus: here

Lab 1Bacterial Transformation/DNA IS the genetic material

Transformation animation here   Transformation discussion handout: here  Great video about GFP: here

Lab 2---Plasmid DNA purification 

Lab 3--Analysis of the pGLO plasmid  Good video on restriction enzymes: HERE (scroll in about 3:50 minutes).

Lab 4--DNA electrophoresis of pGLO plasmid.  Analyzing electrophoresis and contructing a restriction map.

Gel pictures here:

Sara/Laura

Sam/Melissa

Marisol/Sarah

Lauren/Allysa

Jake/Jake

Erin/Wyatt

Brady/Peter

Lab 5---purifying GFP from E. coli transformants.

Lab 6--- pGLO sequence file.  If you want to bring your own computer, please do.  I will have department laptops for your use.

Lab 7---Conventional PCR/Endpoint PCR

Lab 7---DNA agarose gel to analyze PCR

 

OLD MATERIAL BELOW THIS LINE

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ANIMATIONS

 

More terrific animations here!

The Meselson-Stahl Experiment

 

Animation   Essential Biochemistry - DNA Replication

DNA replication 2

Transcription

 

LpGLO sequenc

Lab 7--SDS-PAGE

Lab 8--Endpoint PCR

Lab 8 part 2 agarose gel

Lab 9  real-time PCR

Lab 9--analysis (additional graphs will be given in lab)

 

 

 

Good website for GFP info:http://www.conncoll.edu/ccacad/zimmer/GFP-ww/GFP-1.htm

 

 

 

 

 

 

 

 

 

 

Lecture handouts week of 2-22-10 molecular documents week 3-22-10

 

Molecules of the cell--Reading

GFP readings

lactoferrin gfp  pGLO lactoferrin

walnut GFP.jpg  walnut GFP

 

Lab 1-- PDF lab1   or Word doc lab1transformation2010

Lab 2Lab 2 plasmid pure

Lab3lab3plasmidanalysis

Lab 4lab 4 plasmidanalysis-week 2.doc

Lab 5lab 5 gfp purification

protein gel picture

-Native gel picture from previous  year's class.  Lane 1 is protein marker, the rest of the lanes are lysates from E. coli transformants. Our gel did not run far enough to see many bands or to resolve the molecular weight marker.

protein gel

Lab 6molecular lab 6 2010 part 1

Lab 6 part 2molecular lab 6 2010 part 2

Lab 7lab7 real time PCR

 

 

 

 

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ANIMATIONS

The Meselson-Stahl Experiment

Animation   Essential Biochemistry - DNA Replication

Protein Synthesis Animation

Semi-conservative replication animation

 

DnaTube.com link here--check out protein folding!

DnaTube.com Scientific Video Site

Telomerase demos

Telomerase!

Horn Lab - Projects

 

FORMS

lecuture summary page