Molecular Biology Course Page--FALL 2012 (H. Super)

 Minot State University

Biol 480/ Molecular Biology Course Page

Fall 2014

ANNOUNCEMENTS

 

Check out this awesome resourse:  DNA Learning Center

 

LECTURE MATERIALS

Syllabus 2014

General expectations

Powerpoint lectures:

Lecture 1

Lecture 2

 

LAB MATERIALS

Lab syllabus: here

Lab 1

 

OLD MATERIAL BELOW THIS LINE

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ANIMATIONS

 

More terrific animations here!

The Meselson-Stahl Experiment

 

Animation   Essential Biochemistry - DNA Replication

DNA replication 2

Transcription

 

Lab 1--Bacterial Transformation/DNA IS the genetic material

Lab 1 discussion--be sure to have gone over plates from lab 1 before you come to lab for experiement 2.  I expect group discussion!

Lab 2--PGLO plasmid purification

Lab 3--Using Spectrophotometry to analyze plasmid DNA/Setting up restriction digests of plamid

Lab 4--Analysis of restriction fragments

See your electrophoresis results here.

Lab 5--purification of GFP

Lab 6--Bioinformatics/PCR primer design---I will bring printed information. Please bring your own computer if possible.  

pGLO sequenc

Lab 7--SDS-PAGE

Lab 8--Endpoint PCR

Lab 8 part 2 agarose gel

Lab 9  real-time PCR

Lab 9--analysis (additional graphs will be given in lab)

 

 

 

Good website for GFP info:http://www.conncoll.edu/ccacad/zimmer/GFP-ww/GFP-1.htm

 

 

 

Exam topics lists

 

 

 

 

 

 

Lecture handouts week of 2-22-10 molecular documents week 3-22-10

 

lab 7 posted below

 

Total points at mid term: 222

LECTURE MATERIAL

topics list topicsexam2

molecular 2010 syllabus

Molecules of the cell--Reading

GFP readings

lactoferrin gfp  pGLO lactoferrin

walnut GFP.jpg  walnut GFP

 

 

LAB MATERIAL

Syllabus  MOLECULAR LAB.-lab 2010

Lab 1-- PDF lab1   or Word doc lab1transformation2010

Lab 2Lab 2 plasmid pure

Lab3lab3plasmidanalysis

Lab 4lab 4 plasmidanalysis-week 2.doc

Lab 5lab 5 gfp purification

protein gel picture

-Native gel picture from previous  year's class.  Lane 1 is protein marker, the rest of the lanes are lysates from E. coli transformants. Our gel did not run far enough to see many bands or to resolve the molecular weight marker.

protein gel

Lab 6molecular lab 6 2010 part 1

Lab 6 part 2molecular lab 6 2010 part 2

Lab 7lab7 real time PCR

 

 

 

 

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ANIMATIONS

The Meselson-Stahl Experiment

Animation   Essential Biochemistry - DNA Replication

Protein Synthesis Animation

Semi-conservative replication animation

 

DnaTube.com link here--check out protein folding!

DnaTube.com Scientific Video Site

Telomerase demos

Telomerase!

Horn Lab - Projects

 

FORMS

lecuture summary page