Molecular Biology Course Page--Spring 2016 (H. Super)

 Minot State University

Biol 480/ Molecular Biology Course Page

Spring 2015

 

Aqueorea victoria---the source of the GFP gene in pGLO

ANNOUNCEMENTS

Exam 1 February 8th!

 

Check out these awesome resourses:  

Science Daily (news site)

DNA Learning Center

 

IFL science :)

 

LECTURE MATERIALS

Syllabus 2016

General expectations

Powerpoint lectures:

Lecture 1

Lecture 2

Lecture 3

Lecture 4

Lecture 5

Lecture 6

Lecture 7

Lecture 8

Lecture 9

Lecture 10---will bring to class

Topics Lists for Exams

 

 

LAB MATERIALS

Lab syllabus: here

Lab 1Bacterial Transformation/DNA IS the genetic material

 Transformation animation here   Transformation discussion handout: here  Great video about GFP: here

Lab 2--Purification of pGLO plasmid

 

 

 

 

OLD MATERIAL BELOW THIS LINE

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Lab 3--Analysis of the pGLO plasmid  Good video on restriction enzymes: HERE (scroll in about 3:50 minutes).

Lab 4--DNA electrophoresis of pGLO plasmid.  Analyzing electrophoresis and contructing a restriction map.

Gel pictures here:

Sara/Laura

Sam/Melissa

Marisol/Sarah

Lauren/Allysa

Jake/Jake

Erin/Wyatt

Brady/Peter

Lab 5---purifying GFP from E. coli transformants.

Lab 6--- pGLO sequence file.  If you want to bring your own computer, please do.  I will have department laptops for your use.

Lab 7---Conventional PCR/Endpoint PCR

Lab 7---DNA agarose gel to analyze PCR

Lab 8---real time PCR

Lab 8--real time PCR (qPCR) analysis

Discussion

Data 2

 

 

 

 

ANIMATIONS

 

More terrific animations here!

The Meselson-Stahl Experiment

 

Animation   Essential Biochemistry - DNA Replication

DNA replication 2

Transcription

 

 

Lab 7--SDS-PAGE

Lab 9  real-time PCR

Lab 9--analysis (additional graphs will be given in lab)

 

 

 

Good website for GFP info:http://www.conncoll.edu/ccacad/zimmer/GFP-ww/GFP-1.htm

 

 

 

 

 

 

 

 

 

 

Lecture handouts week of 2-22-10 molecular documents week 3-22-10

 

Molecules of the cell--Reading

GFP readings

lactoferrin gfp  pGLO lactoferrin

walnut GFP.jpg  walnut GFP

 

 

Lab3lab3plasmidanalysis

Lab 4lab 4 plasmidanalysis-week 2.doc

Lab 5lab 5 gfp purification

protein gel picture

-Native gel picture from previous  year's class.  Lane 1 is protein marker, the rest of the lanes are lysates from E. coli transformants. Our gel did not run far enough to see many bands or to resolve the molecular weight marker.

protein gel

Lab 6molecular lab 6 2010 part 1

Lab 6 part 2molecular lab 6 2010 part 2

Lab 7lab7 real time PCR

 

 

 

 

 

 

ANIMATIONS

The Meselson-Stahl Experiment

Animation   Essential Biochemistry - DNA Replication

Protein Synthesis Animation

Semi-conservative replication animation

 

DnaTube.com link here--check out protein folding!

DnaTube.com Scientific Video Site

Telomerase demos

Telomerase!

Horn Lab - Projects

 

FORMS

lecuture summary page