Molecular Biology Course Page--Spring 2019 (H. Super)

 Minot State University

Biol 480/ Molecular Biology Course Page

Spring 2019

 

 

ANNOUNCEMENTS

Check out these awesome resourses:  

Science Daily (news site)

DNA Learning Center

IFL science :)

 

LECTURE MATERIALS

Syllabus 2019

google book 

 

Powerpoint lectures:

Lecture 1

Lecture 2

Lecture 3   Decoding Watson---Documentary

Lecture 4

Lecture 5

Lecture 6  Youtube video on DNA Topology

Lecture 7

Lecture 8

Lecture 9   Epigenetics chapter notes

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Lecture 10--updated

Lecture 11

Lecture 12

Lecture 13  Histones/replication paper

Lecture 14

Lecture 15

Lecture 16

Lecture 17

Lecture 18

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Lecture 19

Lecture 20

Lecture 21

Lecture 22

Lecture 24

Lecture 25

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Lecture 26

Lecture 27

Lecture 28

Lecture 29-part 1

Lecture 29-part 2

Lecture 30

Lecture 31

Lecture 32

 

Topics Lists for Exams

Topics exam 1

Topics exam 2

Topics exam 3

 

 

LAB MATERIALS

Lab syllabus

Lab 1Bacterial Transformation/DNA IS the genetic material   Transformation animation here. Transformation analysis handout: here

Lab 2--- pGLO plasmid purification.   (Be sure to bring your analysis handout from Lab 1 and have hade detailed observations of your 6 plates.  We will start with discussion.

Lab 3---Basic analyis of pGLO plasmid.  Good video on restriction enzymes: HERE (scroll in about 3:50 minutes).

Lab 4--electrophoresis of pGLO plasmid restriction fragments---making a restriction map. Agarose gel prep. Here

Electrophresis animation  http://www.youtube.com/watch?src_vid=0x2Lh5Rq8e0&annotation_id=annotation_299771&feature=iv&v=v0sJRZATXEA

Gel pictures pGLO-gel 1           pGLO gel-2

Lab 5--Bioinformatics using pGLO sequence.   PDFpGLO sequence file.  Word pGLO sequence file. If you want to bring your own computer, please do.  I will have department laptops for your use.

Lab 6--Conventional/endpoint PCR--amplifying GFP sequence using your primers

Lab 6- Part 2/Analyzing endpoint PCR

Lab 7--qPCR (real time PCR).

Lab 8--Purifying GFP protein

Lab 9 --SDS-PAGE. GFP separation using polyacrylamide gel electrophoresis.  AMENDED PROTOCOL

 

 

 

Previous year  MATERIAL BELOW THIS LINE--not current for 2019

 

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Lab 8---Real-time/quantitative PCR

Lab 8--Part 2  (print, read, and bring to lab)

View these docuemnts before lab.  Powerpoint with Ct curves  qPCR data  Standard curves

Lab 6--- pGLO sequence file.  

Lab 8---real time PCR

Lab 8--real time PCR (qPCR) analysis

Discussion

Data 2

 

 

 

 

ANIMATIONS

 

More terrific animations here!

The Meselson-Stahl Experiment

 

 

Transcription

 

 

Lab 7--SDS-PAGE

Lab 9  real-time PCR

Lab 9--analysis (additional graphs will be given in lab)

 

 

 

Good website for GFP info:http://www.conncoll.edu/ccacad/zimmer/GFP-ww/GFP-1.htm

 

 

 

 

 

 

 

 

 

 

Lecture handouts week of 2-22-10 molecular documents week 3-22-10

 

Molecules of the cell--Reading

GFP readings

lactoferrin gfp  pGLO lactoferrin

walnut GFP.jpg  walnut GFP

 

 

Lab3lab3plasmidanalysis

Lab 4lab 4 plasmidanalysis-week 2.doc

Lab 5lab 5 gfp purification

protein gel picture

-Native gel picture from previous  year's class.  Lane 1 is protein marker, the rest of the lanes are lysates from E. coli transformants. Our gel did not run far enough to see many bands or to resolve the molecular weight marker.

protein gel

Lab 6molecular lab 6 2010 part 1

Lab 6 part 2molecular lab 6 2010 part 2

Lab 7lab7 real time PCR

 

 

 

 

 

 

ANIMATIONS

The Meselson-Stahl Experiment

Animation   Essential Biochemistry - DNA Replication

Protein Synthesis Animation

Semi-conservative replication animation

 

DnaTube.com link here--check out protein folding!

DnaTube.com Scientific Video Site

Telomerase demos

Telomerase!

Horn Lab - Projects

 

FORMS

lecuture summary page

AttachmentSize
Biol 480 2014lecture34.ppt2.36 MB
Biol 480 2014lecture35-2.ppt2.39 MB
Biol 480 2014lecture36.ppt3.5 MB
Biol 480 2014lecture37.ppt3.33 MB
Biol 480lecture 35.pptx1.52 MB
Biol 480lecture 35.pptx1.52 MB
Biol 480lecture 36.pptx1.77 MB
Lab8GFPpurification.doc48.5 KB
Lecture 30.pptx5.12 MB