Molecular Biology Course Page--Spring 2016 (H. Super)

 Minot State University

Biol 480/ Molecular Biology Course Page

Spring 2015

 

Aqueorea victoria---the source of the GFP gene in pGLO

ANNOUNCEMENTS

 

 

Check out these awesome resourses:  

Science Daily (news site)

DNA Learning Center

 

IFL science :)

 

LECTURE MATERIALS

Syllabus 2016

General expectations

Powerpoint lectures:

Lecture 1

Lecture 2

Lecture 3

Lecture 4

Lecture 5

Lecture 6

Lecture 7

Lecture 8

Lecture 9

Lecture 10

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Lecture 11

Animation   Essential Biochemistry - DNA Replication

DNA replication 2

Lecture 12

Lecture 13

Lecture 14

Lecture 15

Lecture 16

Lecture 17

Lecture 18

Lecture 19

Lecture 20

Lecture 21

Lecture 22

Lecture 23

Lecture 24

Lecture 25

Lecture 26

Lecture 27

Lecture 28

Lecture 29

Lecture 30

Lecture 31

Lecture 32  Beta galactosidase paper

Lecture 33

Lecture 34

Lecture 35

Lecure 36---I will bring copies to class.

 

 

 

Topics Lists for Exams

Topics list 1

Topics list 2

Topics list 3

Topics list 4

Histones in replication

 

 

LAB MATERIALS

Lab syllabus: here

Lab 1Bacterial Transformation/DNA IS the genetic material

 Transformation animation here   Transformation discussion handout: here  Great video about GFP: here

Lab 2--Purification of pGLO plasmid

Lab 3--Determining concentration of plasmid DNA and quality of PGLO plasmid sample.  Using restriction enzymes.  Good video on restriction enzymes: HERE (scroll in about 3:50 minutes).

Lab 4--pGLO restriction mapping/Electrophoresis and Beyond!

GEL PICTURES!

Emeka/Spencer

Larua/Tess

Chelsey/Erika

Fey/Sydney

Alex/Sam

Lab 5--Bioinformatics---Preparing for PCR with pGLO.  PDFpGLO sequence file.  Word pGLO sequence file If you want to bring your own computer, please do.  I will have department laptops for your use.

Lab 6--Purifying GFP from E. coli transformants

Lab 7---endpoint PCR.  Usinging PCR primers of your design!

Lab 7 part 2---DNA gel electrophoresis---analyzing the PCR amplicon

Lab 8---Real-time/quantitative PCR

Lab 8--Part 2  (print, read, and bring to lab)

View these docuemnts before lab.  Powerpoint with Ct curves  qPCR data  Standard curves

 

 

 

 

 

OLD MATERIAL BELOW THIS LINE

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Lab 3--Analysis of the pGLO plasmid  

Lab 4--DNA electrophoresis of pGLO plasmid.  Analyzing electrophoresis and contructing a restriction map.

 

Lab 5---purifying GFP from E. coli transformants.

Lab 6--- pGLO sequence file.  If you want to bring your own computer, please do.  I will have department laptops for your use.

Lab 7---Conventional PCR/Endpoint PCR

Lab 7---DNA agarose gel to analyze PCR

Lab 8---real time PCR

Lab 8--real time PCR (qPCR) analysis

Discussion

Data 2

 

 

 

 

ANIMATIONS

 

More terrific animations here!

The Meselson-Stahl Experiment

 

 

Transcription

 

 

Lab 7--SDS-PAGE

Lab 9  real-time PCR

Lab 9--analysis (additional graphs will be given in lab)

 

 

 

Good website for GFP info:http://www.conncoll.edu/ccacad/zimmer/GFP-ww/GFP-1.htm

 

 

 

 

 

 

 

 

 

 

Lecture handouts week of 2-22-10 molecular documents week 3-22-10

 

Molecules of the cell--Reading

GFP readings

lactoferrin gfp  pGLO lactoferrin

walnut GFP.jpg  walnut GFP

 

 

Lab3lab3plasmidanalysis

Lab 4lab 4 plasmidanalysis-week 2.doc

Lab 5lab 5 gfp purification

protein gel picture

-Native gel picture from previous  year's class.  Lane 1 is protein marker, the rest of the lanes are lysates from E. coli transformants. Our gel did not run far enough to see many bands or to resolve the molecular weight marker.

protein gel

Lab 6molecular lab 6 2010 part 1

Lab 6 part 2molecular lab 6 2010 part 2

Lab 7lab7 real time PCR

 

 

 

 

 

 

ANIMATIONS

The Meselson-Stahl Experiment

Animation   Essential Biochemistry - DNA Replication

Protein Synthesis Animation

Semi-conservative replication animation

 

DnaTube.com link here--check out protein folding!

DnaTube.com Scientific Video Site

Telomerase demos

Telomerase!

Horn Lab - Projects

 

FORMS

lecuture summary page

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